MORINAGA

RESEARCH CENTER

Dengan pengalaman lebih dari 90 tahun, Morinaga Research Center Japan senantiasa berinovasi dengan meneliti berbagai manfaat ASI dan nutrisi penting pertumbuhan anak. Didukung teknologi yang canggih dan pengalaman yang matang di bidang nutrisi, Morinaga Research Center Japan sangat mengutamakan keamanan dan mutu produk bagi Generasi Platinum.

RISET KAMI

Investigation on the Effects of Lactoferrin-Added Formula on Premature Infants

Showa University Pediatrics Department ; Shigeru Kawaguchi, Takahisa Suzuka, Kazuo Okuyama

The nutritional superiority of breast milk for neonates and infants has been well-known and coupled with advances in manufacturing techniques for powdered formula, various measures have been tried to bring the composition closer to breast milk. However, lactoferrin, which is present in large quantities in breast milk and is one of important factors for the establishment of normal intestinal bacterial flora and prevention of infection is not contained in currently marketed powdered formulas at all. Therefore, we investigated fecal characteristics and bacterial flora in feces of low birth weight infants fed with lactoferrin-added powdered formula, compared with commercial formulas. We herein report some data obtained.

Bacteriostatic Effect of Orally Administered Bovine Lactoferrin on Proliferation of Clostridium Species in the Gut of Mice Fed Bovine Milk

Nutritional Science Laboratory and Biochemical Research Laboratory, Morinaga Milk Industry Co., Ltd., Zama-City, Kanagawa 228, Japan; 1994

When milk-fed mice were orally inoculated with Clostridium ramosum C1, this strain proliferated in the gut and became the dominant component of the fecal microflora. In this experimental model, bovine lactoferrin (bLF) administered with milk suppressed the proliferation of this strain in vivo and decreased the numbers of C. ramosum and other bacteria in the feces. This bacteriostatic effect of bLF was dependent on the concentration of bLF, the duration of feeding, and the administered dose of C. ramosum C1. Compared with bovine serum albumin, ovalbumin, bovine whey protein isolate, or bovine casein, only bLF showed this specific activity. A similar effect of bLF was observed after oral inoculation with C. ramosum JCM 1298, C. paraputrificum VPI 6372, or C. perfringens ATCC 13124. A hydrolysate prepared by digestion of bLF with porcine pepsin showed the same inhibitory effect on proliferation of C. ramosum in vivo as occurred with undigested bLF. These results indicate that ingested bLF can exert a bacteriostatic effect against clostridia in the gut even after it has been digested to some extent

Bovine lactoferrin and lactoferricin derived from milk: production and applications

M. Tomita, H. Wakabayashi, K. Yamauchi, S. Teraguchi, and H. Hayasawa; 2002

Bovine lactoferrin is produced on an industrial scale from cheese whey or skim milk. The safety of purified lactoferrin has been confirmed from the results of a reverse mutation test using bacteria, a 13-week oral repeated-dose toxicity study in rats, and clinical studies. In order to apply active lactoferrin to various products, a process for its pasteurization was developed. Subsequently, lactoferrin has been used in a wide variety of products since it was first added to infant formula in 1986. A pepsin hydrolysate of lactoferrin is also used in infant formula. This hydrolysate contains a potent antimicrobial peptide named lactoferricin that is derived from the lactoferrin molecule by pepsin digestion. Semilarge-scale purification of lactoferricin can be performed by hydrophobic interaction chromatography. Lactoferricin also exhibits several biological actions and appears to be the functional domain of lactoferrin. Recent studies have demonstrated that oral administration of lactoferrin or lactoferricin exerts a host-protective effect in various animals and in humans. The results of these studies strongly suggest that the effects of oral lactoferrin are mediated by modulation of the immune system. Further elucidation of the clinical efficacy and mechanism of action of lactoferrin will increase the value of lactoferrin-containing products.

Lactoferrin research, technology and applications

Hiroyuki Wakabayashi, Koji Yamauchi, Mitsunori Takase; 1999

Lactoferrin is an iron-binding glycoprotein present in milk as well as other exocrine secretions and neutrophil granules in mammals. Lactoferrin is considered to be an important host defense molecule and has a diverse range of physiological functions such as antimicrobial/antiviral activities, immunomodulatory activity, and antioxidant activity. During the past decade, it has become evident that oral administration of lactoferrin exerts several beneficial effects on the health of humans and animals, including anti-infective, anticancer, and anti-inflammatory effects. This has enlarged the application potential of lactoferrin as a food additive. The technology of producing bovine lactoferrin on a factory scale was established over 20 years ago. Bovine lactoferrin is purified by cation-exchange chromatography from bovine skim milk or whey, and is commercially available from several suppliers. Recombinant human lactoferrin is produced by Aspergillus niger, transgenic cows, and rice, and its efficacy is being evaluated. In this article, we review basic research and technological aspects of the application of lactoferrin.

The survival of ingested lactoferrin in the gastrointestinal tract of adult mice

Hidefumi KUWATA , Tai-Tung YIP, Koji YAMAUCHI, Susumu TERAGUCHI, Hirotoshi HAYASAWA, Mamoru TOMITA and T. William HUTCHENS; 1999

Lactoferrin is an 80 kDa major protein component of mammalian colostral whey. The antimicrobial active centre of lactoferrin, lactoferricin (Lfcin), may also be an important determinant of the interaction between lactoferrin and specific receptors on lymphocytes. We have documented the survival in io of ingested lactoferrin in the gastrointestinal tract of adult mice by surfaceenhanced laser desorption}ionization affinity MS. Various kinds of degraded lactoferrin fragments were detected as molecular-ion peaks corresponding to Lfcin after being captured by an affinity capture device, hydrolysis in situ and laser desorption}ionization.

Effects of Lactulose-added Formulae on the Intestinal Flora of Infants from 9 to 12 Months of Age

Hirotoshi Hayasawa, Mitsunori Takase, Kouzou Kawase, Isao Kiyosawa, and Shigeo Okonogi; 1982

The effects of 3 types of formulae on the intestinal flora, certain characteristics of feces and the body weight gain of infants from 9 to 12 months of age were investigated. Formulae used in this study were Infant Formula [L(+)M] and Follow-up Formula [L(+)F] with lactulose and Follow-up Formula [L(-)F] without lactulose.

Effects of Composition of Infant Formulas on the Intestinal Microflora of Infants

KOUZOU KAWASE, Takashi SUZUKI, Isao KIYOSAWA, Shigeo OKONOGI, Takuji KAWASHIMA, and Morio KUBOYAMA; 1983

This review deals with the effects of composition of infant formulas on the intestinal microflora of infants. The addition of bifidus factor, such as lactulose and mucin, works effectively to stimulate the growth of bifidobacteria, lower pH values and raise the lysozyme activity in the feces of bottle-fed infants. Also, low buffering capacity resultant from reducing the protein and phosphorus content in the formula contributes to the predominance of bifidobacteria. These facts indicate that the addition of bifidus factor and low buffering capacity play a significant role to resemble the fecal microflora of bottle-fed infants to that of breast-fed infants through the enhancing multiplication of bifidobacteria, though the ratio of bifidobacteria count to total anaerobic bacteria count in bottle-fed infants is still lower than that in breast-fed infants.

Lactulose and Intestinal Microflora in Infant Nutrition

Isao KIYOSAWA, Mitsunori TAKASE, Koji YAMAUCHI, Joji ONO, Tomoko YAESHIMA and Shigeo OKONOGI; 1985

In breast-fed newborn infants, a stable microflora having more than 90% Bifidobacterium is usually developed in the colon and the feces within 5 days after birth. As a result, pH value lowers and putrefactive bacteria decrease in the feces. In bottle feeding with the milk containing a certain quantity of lactulose, it is observed that pH value, the population of Bifidobacterium, and lysozyme activity in the feces approach the levels of breast feeding. The ratio of Bifidobacterium to the total anaerobic bacteria, lysozyme activity, and concentration of the organic acids were increased while pH was lowered in the feces of infants who were fed the follow-up formula food containing lactulose. Composition of the organic acids in the feces varied depending upon the age. The values of molar ratio of acetic acid to lactic acid in feces of suckling infants were 2.7 to 5.3 and they were elevated by 5- to 7-fold in those feces of weaning infants, while lactic acid was not detected in the feces of the adult subjects. In the in vitro experiments, all bifidobacterial species of the present study assimilated lactulose. The activity for lactulose assimilation was, on the contrary, deficient in Clostridium dificile. When C. perfringens or E. coli was cultured simultaneously with the Bifidobacterium in the PYF culture medium containing lactulose, the former two microbials were suppressed remarkably. Infant rats were inoculated with C. perfringens and fed the milk containing lactulose. The occurrence number of the rats in which C. perfringens was detected in the colon also tends to decline during the feeding

Lactulose and Its Application to the Food and Pharmaceutical Industries

Y. Tamura, T. Mizota, S. Shimamura, and M. Tomita; 1993

We are now in an aging society, with health isseus becoming one of the most important problems. It is expected that a more healthy life in old age may be enjoyed if the intestinal microflora of aged people is kept in good balance. Therefore, it prompts us to believe that the utilization of lactulose not only should be focused on specialized fields, but also should be widely applied to food with the objectives of improving human health and reducing medical costs.

Effect of Feeding Infant Formula Containing Lactulose on Intestinal Flora in the Infant

Y. Tamura, T. Mizota, S. Shimamura, and M. Tomita

It is well known that infants fed on breast milk are better protected from diarrheal disorders fue to the presence of bifidogenic substances in the milk. Many wokrers have shown that bifidobacterial flora are predominant in the stools of babies fed breast milk, while coliforms predominate in those bottlefed. Preponderance of bifidobacteria in the intestine results in many beneficial effects to the host, such as suppression of growth of putrefactive bacteria due to reduction in intestinal pH, better absorption of nutrients, and synthesis of B-complex vitamins.

Prebiotic Bifidobacterial Strains from morinaga Milk Industry Co.,Ltd.

Morinaga Milk Industry Co.,Ltd.

Bifidobacteria is one of the predominant components of human microbiota, especially in infants. Bifidobacteria dominate the microbiota in the intestines of healthy infants fed with breast milk by more than 90%. Bifidobacteria exist at a lower rate in intestines of non-breast milk fed infants. Bifidobacteria function in maintaining health and protecting infants from infection.

Efficacy of Bifidobacterium longum BB536

Morinaga Milk Industry Co.,Ltd.

Tells the effects on human (gastrointestinal, contispation, diarrhea, intestinal environment, prevention on allergy, prevention on the infection, immuno-response, infant health, reducing colesterol), Animal study (cell growth, immuno-stimulation and prevention of infection, bone strength, and immuno-modulation or allergy), and in vitro study (improvement of intestinal condition or suppression of harmful bacteria, immuno-stimulation or immuno-modulation, improvement of cholesterol level, and safety evaluation).

Report On Receiving The Lands Industrial Technology Award from The Japan Society for Lipid Nutrition

2014

We have conducted research into the lipid nutrition of infant formula, and in recent years have studied the nutrition of expectant and nursing mothers, and developed products based on the results. These studies and products were recognized for their contribution to the development of the industrial technology in this field.